Target Finger Modification

Presented data provide first evidence that target finger modification (TFM) strategies are likely to produce zinc finger arrays with better clinical compatibility compared to modular assembly strategies. Results are ready for publication.

Result of EMSA for 2nd finger of Sp1 mutants CB1 and MR14:

Targeted mutations of the second finger of Sp1 produced CB1 and MR14.

We introduced following mutations into 2nd finger of Sp1 targeted PCR mutagenesis (Kits)

target finger modification

Results are ready for publication.

Kcube Analysis of Phosphor Imager Readings CB1

target finger modification 1

Kcube results showing complete recognition code of CB1.

Kcube Analysis of Phosphor Imager Readings MR14

target finger modification 3

Computational Kcube shows complete recognition code of Sp1 mutant MR14.